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ABflo® 488 Rabbit anti-Human CD185/CXCR5 mAb (100 T)
| Reactivity: | Human |
| Applications: | FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal Antibody |
| Conjugation: | ABflo® 488. Ex:491nm. Em:516nm. |
| Gene Name: | C-X-C motif chemokine receptor 5 |
| Gene Symbol: | CXCR5 |
| Synonyms: | BLR1; CD185; MDR15 |
| Gene ID: | 643 |
| UniProt ID: | P32302 |
| Clone ID: | 9C6P8 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-47aa of Human CD185/CXCR5 (NP_033981.2). |
| Dilution: | FC,5 μl per 10^6 cells in 100 μl volume |
| Purification Method: | Affinity purification |
| Concentration: | 0.02 mg/mL |
| Buffer: | PBS with 0.03% proclin300,0.2% BSA, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-CXCR5 Antibody |
Background
This gene encodes a multi-pass membrane protein that belongs to the CXC chemokine receptor family. It is expressed in mature B-cells and Burkitt's lymphoma. This cytokine receptor binds to B-lymphocyte chemoattractant (BLC), and is involved in B-cell migration into B-cell follicles of spleen and Peyer patches. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Images
 | Flow cytometry: 1×10^6 HeLa cells (negative control,left) and Daudi cells (right) were surface-stained with ABflo® 488 Rabbit anti-Human CD185/CXCR5 mAb (A24676,5 μl/Test,orange line) or ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line). |
 | Flow cytometry: 1×10^6 Daudi cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD185/CXCR5 mAb (A24676,5 μl/Test,right). |
 | Flow cytometry: 1×10^6 Human PBMC were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD185/CXCR5 mAb (A24676,5 μl/Test,right). |
 | Flow cytometry:1×10^6 Human PBMC were surface-stained with ABflo® 647 Mouse anti-Human CD19 mAb (5 μl/Test) and ABflo® 488 Rabbit IgG isotype control (A22069,5 μl/Test,left) or ABflo® 488 Rabbit anti-Human CD185/CXCR5 mAb (A24676,5 μl/Test,right). Cells in the lymphocyte gate were used for analysis. |
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